Select Microorganism Type

Azospirillum brasilense #858

Generality
Species Azospirillum brasilense 
Isolated by Mazzotti F., Landi S. 
Identified by Castaldini M., Mazzotti F., Landi S. 
Accession Number CRA - ABP #858 
Year 2006 
State Italia 
Region (regione) Basilicata 
District (provincia) Potenza 
Isolated from Soil + temporary grasslland + conentional manageme 
Location Viggiano 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium PY (Antibiotic medium n° 3 DIFCO) 
Year 2008 
N° of replicate maintained
Characterization
Molecular characterization
16S rDNA sequence  GenBank accession number EF634031
nifH gene partial sequence  GenBank accession number EF634049
Molecular markers
ARDRA Fragments  430, 330, 210, 180, 80, 60 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 140, 40 (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
Supply
Supply Active