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Azospirillum brasilense #857

Species Azospirillum brasilense 
Isolated by Mazzotti F., Landi S. 
Identified by Castaldini M., Mazzotti F., Landi S. 
Accession Number CRA - ABP #857 
Year 2006 
State Italia 
Region (regione) Basilicata 
District (provincia) Potenza 
Isolated from Soil + tempotary grassland + conventional manageme 
Location Viggiano 
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium PY (Antibiotic medium n° 3) 
Year 2008 
N° of replicate maintained
Molecular characterization
16S rDNA sequence  GenBank accession number EF634040
nifH gene partial sequence  GenBank accession number EF634048
Molecular markers
ARDRA Fragments 430, 330, 210, 180, 80, 60 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 140, 40 (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
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