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Paenibacillus polymyxa # 851

Species Paenibacillus polymixa 
Isolated by Mazzotti F., Landi S. 
Identified by Castaldini M., Mazzotti F., Landi S. 
Accession Number CRA - ABP #851 
Year 2005 
State Italia 
Region (regione) Basilicata 
District (provincia) Potenza 
Isolated from Soil with Hordeum vulgare and temporary grassland 
Location Viggiano 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium TSB + Yeast extract 0,5% 
Year 2008 
N° of replicate maintained
Molecular characterization
16S rDNA sequence  GenBank accession number EF634024
nifH gene partial sequence  GenBank accession number EF634042
Molecular markers
ARDRA Fragments 410, 330, 240, 180 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 318, 42 (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
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