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Rhizobium gallicum # 850

Generality
Species Rhizobium gallicum 
Isolated by Mazzotti F., Landi S. 
Identified by Castaldini M., Mazzotti F., Landi S. 
Accession Number CRA - ABP #850 
Year 2005 
State Italia 
Region (regione) Basilicata 
District (provincia) Potenza 
Isolated from soil with barley and temporary grassland 
Location Viggiano 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 2008 
N° of replicate maintained
Characterization
Molecular characterization
16S rDNA sequence GenBank accession number EF634023
nifH gene partial sequence  GenBank accession number EF634041
Molecular markers
ARDRA Fragments 330, 280, 180, 140, 120, 80, 70 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 240, 130, 50 (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
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