Select Microorganism Type

Agrobacterium tumefaciens #827

Generality
Species Agrobacterium tumefaciens 
Isolated by Castaldini M., Landi S., Miclaus N. 
Identified by Castaldini M., Landi S., Fabiani A. 
Accession Number CRA - ABP #827 
Year 2000 
State Italia 
Region (regione) Toscana 
District (provincia) Firenze 
Isolated from 25 year Vineyard 
Location Pontassieve 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 2008 
N° of replicate maintained
Characterization
Molecular characterization
16S rDNA sequence GeneBank accession number EF620473
nifH gene partial sequence GeneBank accession number EF620520
Molecular markers
ARDRA Fragments 346, 291, 181, 148, 122, 77 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 186, 134, 46 (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
Supply
Supply Active