Select Microorganism Type

Pseudomonas putida #590

Generality
Species Pseudomonas putida 
Isolated by Castaldini M., Landi S., Miclaus N.,  
Identified by Castaldini M., Landi S., Miclaus N.,  
Accession Number CRA - ABP #590 
Year 2001 
State Italia 
Region (regione) Veneto 
District (provincia) Verona 
Isolated from Fluventic Xerocrept with Nicotiana tabacum 
Location Bovolone 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 10/05/2003 
N° of replicate maintained
Characterization
Molecular characterization
16S rDNA sequence  GenBank accession number EF620456
nifH gene partial sequence  GenBank accession number EF620503
Molecular markers
ARDRA Fragments 423, 373, 291, 259, 213 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 182, 136, 48, 38 (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
Others Acetylene Reduction Assay 
Supply
Supply Active