Select Microorganism Type

Azotobacter vinelandii #436

Generality
Species Azotobacter vinelandii 
Isolated by Castaldini M., Landi S., Miclaus N.,  
Identified by Castaldini M., Landi S., Miclaus N.,  
Accession Number CRA - ABP #436 
Year 2001 
State Italia 
Region (regione) Marche 
District (provincia) Ascoli Piceno 
Isolated from Beta vulgaris 
Location Carassai 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 2008 
N° of replicate maintained
Characterization
Biological and biochemical characterization
Acetylene Reduction Assay 
Molecular characterization
16S rDNA sequence  GenBank accession number EF620452
nifH gene partial sequence  GenBank accession number EF620501
Molecular markers
ARDRA Fragments 426, 300, 290, 261, 163, 129, (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 183, 137, 50 (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
Supply
Supply Active