Select Microorganism Type

Pseudomonas fluorescens #433

Generality
Species Pseudomonas fluorescens 
Isolated by Castaldini M., Landi S., Miclaus N. 
Identified by Castaldini M., Landi S., Miclaus N. 
Accession Number CRA - ABP #433 
Year 2001 
State Italia 
Region (regione) Marche 
District (provincia) Ascoli Piceno 
Isolated from Calcaric Regosol with Beta vulgaris 
Location Carassai 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 2008 
N° of replicate maintained
Characterization
Biological and biochemical characterization
Acetylene Reduction Assay 
Molecular characterization
ARDRA: 16S rDNA fragment from 8 to 1492 bp digested CfoI
RFLP of partial nifH sequence amplified according to Poly et al., AEM 2001, digested CfoI
ARDRA fragments (bp): 426, 298, 188, 163, 129, 74
nifH RFLP fragments (bp): 183, 138, 74
Molecular markers
ARDRA Fragments 426, 298, 188, 163, 129, 74 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 183, 138, 74 (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
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