Select Microorganism Type

Azotobacter vinelandii #430

Generality
Species Azotobacter vinelandii 
Isolated by Castaldini M., Landi S., Miclaus N. 
Identified by Castaldini M., Landi S., Miclaus N. 
Accession Number CRA - ABP #430 
Year 2001 
State Italia 
Region (regione) Marche 
District (provincia) Ascoli Piceno 
Isolated from Calcaric Regosol with Beta vulgaris 
Location Carassai 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 2008 
N° of replicate maintained
Characterization
Molecular characterization
16S rDNA sequence  GenBank accession number EF620449
nifH gene partial sequence  GenBank accession number EF620498
Molecular markers
ARDRA Fragments 435, 289, 280, 191, 165, 130 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 181, 135, 49, (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
Supply
Supply Active