Select Microorganism Type

Azotobacter vinelandii #428

Generality
Species Azotobacter vinelandii 
Isolated by Castaldini M., Landi S., Miclaus N.ISSDS 
Identified by Castaldini M., Landi S., Miclaus N. 
Accession Number CRA - ABP #428 
Year 2001 
State Italia 
Region (regione) Marche 
District (provincia) Ascoli Piceno 
Isolated from Calcaric Regosol with Beta vukgaris 
Location Carassai 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 2008 
N° of replicate maintained
Characterization
Molecular characterization
16S rDNA sequence  GenBank accession number EF620447
nifH gene partial sequence  GenBank accession number EF620496
Molecular markers
ARDRA Fragments 430, 298, 291, 260, 165, 130 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 181, 135, 49 (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
Supply
Supply Active