Select Microorganism Type

Azotobacter chroococcum #397

Generality
Species Azotobacter chroococcum 
Isolated by Castaldini M., Landi S., Miclaus N. 
Identified by Castaldini M., Landi S., Miclaus N. 
Accession Number CRA - ABP #397 
Year 2001 
State Italia 
Region (regione) Veneto 
District (provincia) Verona 
Isolated from Fluventic Xerochrept with Triticum  
Location Bovolone 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 2008 
N° of replicate maintained
Characterization
Biological and biochemical characterization
Acetylene Reduction Assay 
Molecular characterization
16S rDNA sequence  GenBank accession number EF620440
nifH gene partial sequence  GenBank accession number EF620491
Molecular markers
ARDRA Fragments 579, 423, 289, 259 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 185, 139, 48 (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
Supply
Supply Active