Select Microorganism Type

Azotobacter vinelandii #386

Generality
Species Azotobacter vinelandii 
Isolated by Castaldini M., Landi S., Miclaus N.,  
Identified by Castaldini M., Landi S., Miclaus N.,  
Accession Number CRA - ABP #386 
Year 2001 
State Italia 
Region (regione) Toscana 
District (provincia) Pisa 
Isolated from Vertic Cambis with Sulla , Festuca, Trifolium 
Location Vicarello 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 10/05/2003 
N° of replicate maintained
Characterization
Molecular characterization
16S rDNA sequence  GenBank accession number EF620439
nifH gene partial sequence  GenBank accession number EF620490
Molecular markers
ARDRA Fragments 406, 284, 183, 156, 70 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 184, 138, 46, (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
Supply
Supply Active