Select Microorganism Type

Azotobacter chroococcum #356

Species Azotobacter chroococcum 
Isolated by Castaldini M., Landi S., Miclaus N., 
Identified by Castaldini M., Landi S., Miclaus N., 
Accession Number CRA - ABP #356 
Year 2001 
State Italia 
Region (regione) Toscana 
District (provincia) Pisa  
Isolated from Vertic Cambis with Ulmus Prunus Crataegus Spartium 
Location Vicarello 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 2008 
N° of replicate maintained
Biological and biochemical characterization
Acetylene Reduction Assay 
Molecular characterization
16S rDNA sequence  GenBank accession number EF 620428
nifH gene partial sequence  GenBank accession number EF620479
Molecular markers
ARDRA Fragments 585, 423, 291, 261, (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 184, 139, 49, (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
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