Select Microorganism Type

Azotobacter chroococcum #347

Generality
Species Azotobacter chroococcum 
Isolated by Castaldini M., Landi S., Miclaus N. 
Identified by Castaldini M., Landi S., Miclaus N. 
Accession Number CRA - ABP #347 
Year 2001 
State Italia 
Region (regione) Toscana 
District (provincia) Pisa 
Isolated from Vertic Cambisol with Triticum durum var Grazia 
Location Vicarello 
Place of maintenance Centro di Ricerca per l'Agrobiologia e la Pedologia
Mainteinance Method
Mainteinance Method
frozen (-80 °C)frozen (-80 °C)
Growing method/medium RM (Robson et al., 1984) 
Year 2008 
N° of replicate maintained
Characterization
Biological and biochemical characterization
Acetylene Reduction Assay 
Molecular characterization
16S rDNA sequence  GenBank accession number EF620427
nifH gene partial sequence  GenBank accession number EF620478
Molecular markers
ARDRA Fragments 579, 419, 288, 259 (8 to 1492 bp E.coli position amplification, CfoI digestion)
nifH gene partial sequence RFLP 184, 139, 49, (115 to 476 bp A. vinelandii position amplification, CfoI digestion)
Supply
Supply Active